Class II A2 Biosafety Cabinet YR0090C-SS
Personnel · product · environment · triple protection with HEPA H14 filtration
The Kalstein YR0090C-SS is a Class II Type A2 biosafety cabinet designed for microbiological and biomedical work with BSL-1 to BSL-3 agents. HEPA H14 filters retain 99.995% of particles ≥ 0.3 µm; 70% of the air is recirculated and 30% exhausted, protecting the operator, the sample and the environment simultaneously.
Interactive tour · Explore the YR0090C-SS
Every component, engineered for containment
Six precision-engineered systems working together — press play for a guided tour or click any point on the cabinet to inspect it in detail.
Inspectable components
- Dual HEPA filtrationAir filtration
Supply and exhaust HEPA filtration retains particles ≥ 0.3 µm for Class II A2 containment.
- Digital control panelControl
Fan, UV lamp and LED lighting are operated from one digital interface.
- UV disinfection lampDecontamination
Germicidal UV supports chamber decontamination between operations.
- LED work lightingLighting
Waterproof LED lamps provide uniform visibility across the work zone.
- Tempered glass sashSafety
The front sash works as a physical barrier between operator and samples.
- AISI 304 work zoneMaterials
Stainless work surface designed for cleaning and decontamination.
Key features
- Class II Type A2 configuration certified for BSL-1 to BSL-3 applications
- HEPA H14 downflow and exhaust filters — 99.995% retention at 0.3 µm
- Downflow 0.33 m/s and inflow 0.55 m/s for stable laminar containment
- Stainless steel AISI 304 work chamber with rounded corners for easy cleaning
- Motorized front sash with safe-height alarm and interlock
- Microprocessor controller with real-time airflow monitoring and audible alarms
Triple protection · engineered layers
Personnel protection
Inflow curtain at 0.55 m/s prevents aerosols from escaping the work zone, isolating the operator from BSL-1 to BSL-3 agents.
- Inflow barrier
- Front sash interlock
- Audible alarms
- Inflow velocity
- 0.55 m/s
- Face velocity
- ≥ 100 fpm
- Max biosafety level
- BSL-3
Product protection
HEPA-filtered downflow at 0.33 m/s bathes the sample in ISO 5 laminar air, keeping the culture free from environmental contamination.
- Downflow laminar
- ISO 5 work zone
- HEPA H14 downflow
- Downflow velocity
- 0.33 m/s
- Work zone class
- ISO 5
- Retention @ 0.3 µm
- 99.995%
Environmental protection
30% of the air is exhausted through a second HEPA H14 filter, releasing only clean air back into the laboratory or ducted exhaust.
- HEPA H14 exhaust
- Top duct connection
- Room air quality preserved
- Exhaust ratio
- 30%
- Exhaust filtration
- HEPA H14
- Noise level
- ≤ 62 dB
Airflow modes · what happens in every direction
- Downflow — HEPA downflow at 0.33 m/s
Vertical laminar air descends from the supply HEPA over the entire work zone, keeping the sample under ISO 5 conditions.
- Inflow — Inflow curtain at 0.55 m/s
Room air enters through the front grille at 0.55 m/s, creating an air barrier that stops aerosols from escaping.
- Exhaust — HEPA exhaust through the top duct
30% of the internal air is HEPA H14 filtered and released through the top duct.
- Full cycle — Simultaneous triple protection
Downflow, inflow and exhaust combine to protect personnel, product and environment at the same time.
Technical specifications
| Model | YR0090C-SS |
|---|---|
| Type | Class II Type A2 |
| Filtration | HEPA H14 (99.995% @ 0.3 µm) |
| Downflow velocity | 0.33 m/s |
| Inflow velocity | 0.55 m/s |
| Exhaust distribution | 30% exhaust · 70% recirculation |
| External dimensions | 1350 × 800 × 2250 mm |
| Work chamber | 1130 × 590 × 660 mm |
| Noise level | ≤ 62 dB |
| Illumination | ≥ 1000 lux |
| Power supply | 220 V / 50-60 Hz · 550 W |
Applications
- Microbiology and cell culture
- Virology and BSL-3 research
- Clinical diagnostics and IVF
- Pharmaceutical compounding
- Molecular biology and PCR setup
Real-world use cases · guided workflows
Cell culture and tissue work
Sterile cell culture in a Class II Type A2 environment for cell lines, primary cultures and tissue processing.
- Sanitize the work surface with 70% ethanol
- Start the cabinet and wait for stable airflow
- Transfer materials through the front opening
- Work in the front-to-back laminar zone
- Decontaminate before shutdown
Molecular biology and PCR setup
Prepare PCR reactions and molecular assays free from cross-contamination.
- UV decontaminate for 15 minutes
- Load pipettes, tips and reagents
- Prepare master mix in the clean zone
- Aliquot into reaction tubes
- Clean and log the session
BSL-3 pathogen handling
Manipulation of high-risk agents when integrated in a BSL-3 laboratory suite.
- Verify certification and airflow reports
- Don PPE and follow SOPs
- Introduce material via pass-through
- Perform the procedure inside the containment
- Autoclave waste and decontaminate the chamber
Ideal for applications in
Where the YR0090C-SS performs
Explore each field the YR0090C-SS was engineered for — click any industry to reveal the specific benefits, key metrics and how the cabinet supports the workflow.
Microbiology laboratories
Culture handling, colony isolation and pathogen manipulation under sterile conditions.
key benefits
- Sterile culture handling
- Colony isolation & sub-culturing
- Pathogen manipulation up to BSL-2
- Cleanliness
- ISO 5
- Downflow
- 0.45 m/s
- Continuous
- 24/7
Biotechnology & research
Cell culture, molecular biology and genetic engineering with sample-to-operator isolation.
key benefits
- Primary & stable cell lines
- Transfection / transduction
- CRISPR & genetic engineering
- LED lighting
- 2×10 W
- Stainless
- AISI 304
- Filtration
- Dual HEPA
Pharmaceutical industry
Sterile compounding, formulation R&D and QC of aseptic preparations.
key benefits
- USP 797 aseptic compounding
- Chemotherapy admixtures
- Small-batch sterile filling
- Tipo A2
- Class II
- Recirc / exhaust
- 70/30
- Stainless
- AISI 304
Clinical & diagnostic labs
Processing of blood, tissue and clinical specimens with biological containment.
key benefits
- Blood & serum processing
- Tissue & biopsy handling
- Molecular diagnostics prep
- Containment
- BSL-2
- Decontamination
- UV lamp
- @ 0.3 µm
- ≥99.999%
Biohazardous materials
Safe manipulation of BSL-2 agents, low-volatility chemicals and infectious samples.
key benefits
- BSL-2 infectious agents
- Trace volatile chemicals
- Radionuclide trace work
- HEPA exhaust
- 30%
- Tempered glass
- 5 mm
- Plenum
- Sealed
Fast quotation · 15 seconds
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Frequently asked questions
What biosafety level does the YR0090C-SS cover?
It is certified for BSL-1, BSL-2 and BSL-3 microbiological work when installed and validated according to EN 12469 / NSF 49 protocols.
Is it a Class II Type A2 cabinet?
Yes. 70% of the internal air is HEPA-recirculated and 30% is HEPA-exhausted through the top duct, which is the defining feature of a Class II Type A2 configuration.
What filtration does it use?
HEPA H14 filters on both downflow and exhaust, retaining ≥ 99.995% of particles of 0.3 µm — the reference size for aerosol containment.
Can it be validated on site?
Yes. Kalstein provides commissioning, particle counting and airflow validation services aligned with EN 12469 and NSF/ANSI 49.